Overexpression of tomato SlTpx improves salt stress tolerance in transgenic tobacco plants by scavenging H2O2

Hydrogen peroxide (H2O2) is an important signaling molecule that involved in multiple physiological metabolic processes plants. Excess H2O2 can destroy biological macromolecules to poison the cell. Thioredoxin peroxidase (Tpx) plays role protecting plants from oxidative damage by clearing H2O2. In this study, tomato Tpx (SlTpx) gene was cloned and bioinformatic analysis done. The mRNA transcript level of SlTpx root leaf increased significantly after NaCl stress treatment for 12 h. overexpression transgenic tobacco were obtained study its function under stress. seed germination rate higher than wild type (WT) treatment. malondialdehyde (MDA) content reactive oxygen species (ROS) accumulation less WT Transgenic had antioxidant enzyme activities, proline total soluble sugar contents, expression Na+ metabolism genes WT. Moreover, seeds showed tolerance methyl viologen (MV) treatment, compared with Besides, growth prokaryotic strain pET-28a-SlTpx better pET-28a above results indicate improves plant salt scavenging Overexpression enhances tolerance.